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| = Biology = | | = Biology = |
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| + | * Enzymatic DNA Synthesis Methods, '''Lead: Michael Uttmark''' {{slack-user|uttmark}} |
| + | ** Test commercial blocking groups for compatibility with [[Terminal Deoxynucleotidyl Transferase]] |
| + | ** Chemically synthesize nucleotides with different reversible blocking groups |
| + | ** Characterize and optimize [[Enzymatic Synthesis Methods | enzymatic DNA synthesis]] reaction efficiency |
| + | ** Research purification methods for synthesized DNA |
| + | ** Design and test your own synthesis method! |
| + | * Sequence Verification |
| + | ** Execute and optimize any one of our existing verification procedures--[[Polyacrylamide Gel Electrophoresis]], [[Pyrosequencing]], or [[Ligation and Sequencing]] |
| + | ** Adapt LCMS or MALDI-TOF procedures for detecting single-base addition or determining the sequence of a sample. |
| + | ** Come up with new ways to verify single-base addition to a starting strand of DNA |
| + | * Microfluidic Device Design |
| + | ** Design and program an [[Electrowetting on Dielectric]] microfluidic PCB |
| + | ** Simulate and test how a microfluidic system would work in microgravity |
| + | ** Port our DNA synthesis method to a solid substrate like controlled pore glass or streptavidin-biotin magnetic beads |
| + | ** Optimize an integrated microfluidic protocol for DNA synthesis and verification on the electrowetting PCB and on the [[Beckman Biomek 2000]] liquid handling robot in lab |
| + | ** Research and test other automated [https://en.wikipedia.org/wiki/Microfluidics fluid handling methods], like [https://en.wikipedia.org/wiki/Acoustic_droplet_ejection acoustic droplet ejection] or [https://en.wikipedia.org/wiki/Optoelectrowetting optoelectrowetting]. |
| + | ** Build a system for cooling and temperature control of the device, perhaps using [https://en.wikipedia.org/wiki/Thermoelectric_cooling Peltiers] |
| + | ** Figure out how to power our PCB from a cubesat or other launch vehicle |
| + | ** Build testing rigs for DNA synthesis methods that are needed for experiments in lab |
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| = Policy = | | = Policy = |