Difference between revisions of "Dna synthesizer"
(Created page with "Biology Team's Pilot Project ==Biology Team's 2016 Pilot Project== ==Components of the Synthesis Project== ===Enzymatic Synthesis Chemistry=== ===Safe Phosphoramidite Chemi...") |
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Biology Team's Pilot Project | Biology Team's Pilot Project | ||
| − | == | + | ==SSI Bio 2016 Pilot Project== |
| + | SSI Bio is launching with the DNA synthesizer as their primary project. | ||
==Components of the Synthesis Project== | ==Components of the Synthesis Project== | ||
| + | SSI Bio will be breaking up this DNA synthesizer project into several smaller subteams to tackle the several different systems needed | ||
| + | ===Enzymatic Synthesis Chemistry=== | ||
| − | |||
===Safe Phosphoramidite Chemistry=== | ===Safe Phosphoramidite Chemistry=== | ||
| + | A more conventional alternative to aiming for enzymatic synthesis would be to try to adjust conventional phosphoramidite chemistry to be safer for use in space. This might involve creating a system that can synthesize DNA using safer organic solvents and less toxic reagents. | ||
===Microfluidic Synthesizer Design=== | ===Microfluidic Synthesizer Design=== | ||
===Reassembly Chemistry and Algorithm=== | ===Reassembly Chemistry and Algorithm=== | ||
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====Overview==== | ====Overview==== | ||
Once a strand of DNA is made, we will need to check to make sure that it is the correct desired sequence. | Once a strand of DNA is made, we will need to check to make sure that it is the correct desired sequence. | ||
| − | + | ==Effects of limits of Space on Synthesizer== | |
| − | + | ===Payload Size and Power constraints=== | |
| + | We'd like to fit our synthesizer into a 10 centimeter cube, so that it could be launched on a [https://en.wikipedia.org/wiki/CubeSat CubeSat] or other standardized research payload. | ||
| − | + | ===What kind of shielding do we need?=== | |
| + | We're not sure what kind of shielding we need! UV radiation can have damage DNA through a process called [https://en.wikipedia.org/wiki/Direct_DNA_damage Direct DNA damage] that can lead to thymine or pyrimidine dimers. This is what causes sunburn, and it's why your skin can tan to help block out UVB. | ||
====Communication from Device==== | ====Communication from Device==== | ||
Revision as of 05:35, 17 October 2016
Biology Team's Pilot Project
SSI Bio 2016 Pilot Project
SSI Bio is launching with the DNA synthesizer as their primary project.
Components of the Synthesis Project
SSI Bio will be breaking up this DNA synthesizer project into several smaller subteams to tackle the several different systems needed
Enzymatic Synthesis Chemistry
Safe Phosphoramidite Chemistry
A more conventional alternative to aiming for enzymatic synthesis would be to try to adjust conventional phosphoramidite chemistry to be safer for use in space. This might involve creating a system that can synthesize DNA using safer organic solvents and less toxic reagents.
Microfluidic Synthesizer Design
Reassembly Chemistry and Algorithm
DNA Product Verification
Overview
Once a strand of DNA is made, we will need to check to make sure that it is the correct desired sequence.
Effects of limits of Space on Synthesizer
Payload Size and Power constraints
We'd like to fit our synthesizer into a 10 centimeter cube, so that it could be launched on a CubeSat or other standardized research payload.
What kind of shielding do we need?
We're not sure what kind of shielding we need! UV radiation can have damage DNA through a process called Direct DNA damage that can lead to thymine or pyrimidine dimers. This is what causes sunburn, and it's why your skin can tan to help block out UVB.
Communication from Device
Depending on our ultimate choice of payload, communication from our device involves a number of interesting questions. How do we return the message that synthesis has been carried out successfully? Will the message describe the sequence of the product created, or a simple boolean yes or no?
History of DNA synthesizer idea in SSI
The idea of a DNA synthesizer for space has been floating around SSI for some time. One of the earliest recordings stretches back to 2013, in a talk given John Cumbers. John Cumbers was also consulted during the initial conception and planning of the project in the summer of 2015.