Changes

==Sequencing==

==Sequencing==

Once the DNA sample is purified to remove any remaining nucleotides or enzyme, the circularized DNA can be sent for sequencing by a commercial sequencing service. The primer used to sequence the DNA should be a reverse primer taken from ~200 nt downstream of the 5' end of the original linear starting DNA. The sequencing results should reveal which bases were added and give a rough quantification of addition efficiency.

Once the DNA sample is purified to remove any remaining nucleotides or enzyme, the circularized DNA can be sent for sequencing by a commercial sequencing service. The primer used to sequence the DNA should be a reverse primer taken from ~200 nt downstream of the 5' end of the original linear starting DNA. The sequencing results should reveal which bases were added and give a rough quantification of addition efficiency.