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− | Biology Team's Pilot Project
| + | #REDIRECT [[DNA Synthesizer]] |
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− | ==SSI Bio 2016 Pilot Project==
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− | SSI Bio is launching with the DNA synthesizer as their primary project.
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− | ==Components of the Synthesis Project==
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− | SSI Bio will be breaking up this DNA synthesizer project into several smaller subteams to tackle the several different systems needed
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− | ===Enzymatic Synthesis Chemistry===
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− | ===Safe Phosphoramidite Chemistry===
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− | A more conventional alternative to aiming for enzymatic synthesis would be to try to adjust conventional phosphoramidite chemistry to be safer for use in space. This might involve creating a system that can synthesize DNA using safer organic solvents and less toxic reagents.
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− | ===Microfluidic Synthesizer Design===
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− | ===Reassembly Chemistry and Algorithm===
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− | ===DNA Product Verification===
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− | ====Overview====
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− | Once a strand of DNA is made, we will need to check to make sure that it is the correct desired sequence.
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− | ==Effects of limits of Space on Synthesizer==
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− | ===Payload Size and Power constraints===
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− | We'd like to fit our synthesizer into a 10 centimeter cube, so that it could be launched on a [https://en.wikipedia.org/wiki/CubeSat CubeSat] or other standardized research payload.
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− | ===What kind of shielding do we need?===
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− | We're not sure what kind of shielding we need! UV radiation can have damage DNA through a process called [https://en.wikipedia.org/wiki/Direct_DNA_damage Direct DNA damage] that can lead to thymine or pyrimidine dimers. This is what causes sunburn, and it's why your skin can tan to help block out UVB.
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− | ====Communication from Device====
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− | Depending on our ultimate choice of payload, communication from our device involves a number of interesting questions. How do we return the message that synthesis has been carried out successfully? Will the message describe the sequence of the product created, or a simple boolean yes or no?
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− | ==History of DNA synthesizer idea in SSI==
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− | The idea of a DNA synthesizer for space has been floating around SSI for some time. [[John Cumbers - Synthetic Biology | One of the earliest recordings stretches back to 2013, in a talk given John Cumbers.]] John Cumbers was also consulted during the initial conception and planning of the project in the summer of 2015.
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