Changes

no edit summary
Line 10: Line 10:  
==Sequencing==
 
==Sequencing==
 
Once the DNA sample is purified to remove any remaining nucleotides or enzyme, the circularized DNA can be sent for sequencing by a commercial sequencing service. The primer used to sequence the DNA should be a reverse primer taken from ~200 nt downstream of the 5' end of the original linear starting DNA. The sequencing results should reveal which bases were added and give a rough quantification of addition efficiency.
 
Once the DNA sample is purified to remove any remaining nucleotides or enzyme, the circularized DNA can be sent for sequencing by a commercial sequencing service. The primer used to sequence the DNA should be a reverse primer taken from ~200 nt downstream of the 5' end of the original linear starting DNA. The sequencing results should reveal which bases were added and give a rough quantification of addition efficiency.
 +
 +
[[Category:Biology]]
95

edits