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1,908 bytes added ,  15:39, 19 September 2017
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= Biology =
 
= Biology =
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* Enzymatic DNA Synthesis Methods, '''Lead: Michael Uttmark''' {{slack-user|uttmark}}
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** Test commercial blocking groups for compatibility with [[Terminal Deoxynucleotidyl Transferase]]
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** Chemically synthesize nucleotides with different reversible blocking groups
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** Characterize and optimize [[Enzymatic Synthesis Methods | enzymatic DNA synthesis]] reaction efficiency
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** Research purification methods for synthesized DNA
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** Design and test your own synthesis method!
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* Sequence Verification
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** Execute and optimize any one of our existing verification procedures--[[Polyacrylamide Gel Electrophoresis]], [[Pyrosequencing]], or [[Ligation and Sequencing]]
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** Adapt LCMS or MALDI-TOF procedures for detecting single-base addition or determining the sequence of a sample.
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** Come up with new ways to verify single-base addition to a starting strand of DNA
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* Microfluidic Device Design
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** Design and program an [[Electrowetting on Dielectric]] microfluidic PCB
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** Simulate and test how a microfluidic system would work in microgravity
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** Port our DNA synthesis method to a solid substrate like controlled pore glass or streptavidin-biotin magnetic beads
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** Optimize an integrated microfluidic protocol for DNA synthesis and verification on the electrowetting PCB and on the [[Beckman Biomek 2000]] liquid handling robot in lab
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** Research and test other automated [https://en.wikipedia.org/wiki/Microfluidics fluid handling methods], like [https://en.wikipedia.org/wiki/Acoustic_droplet_ejection acoustic droplet ejection] or [https://en.wikipedia.org/wiki/Optoelectrowetting optoelectrowetting].
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** Build a system for cooling and temperature control of the device, perhaps using [https://en.wikipedia.org/wiki/Thermoelectric_cooling Peltiers]
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** Figure out how to power our PCB from a cubesat or other launch vehicle
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** Build testing rigs for DNA synthesis methods that are needed for experiments in lab
    
= Policy =
 
= Policy =
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