Changes

Jump to navigation Jump to search
64 bytes added ,  06:45, 12 November 2017
no edit summary
Line 5: Line 5:  
[[File:spinner.jpg|350px|thumb|right|Durand Spin Coater in all it's pre-1980's glory]]
 
[[File:spinner.jpg|350px|thumb|right|Durand Spin Coater in all it's pre-1980's glory]]
 
==Permissions==
 
==Permissions==
Anyone who wants to use the spin coater has to contact Michael Arcidiacono ({{slack-user|mixarcid}}) or one of the bio team coleads first. The spin coater is a new addition to the SSI toolset, and no standardized access has been set up as of yet. It's ''essential'' to contact the appropriate personnel before using the spin coater: we want to keep our Durand access!
+
Anyone who wants to use the spin coater has to contact Michael Arcidiacono ({{slack-user|mixarcid}}) or one of the bio team coleads first. The spin coater is a new addition to the SSI toolset and no standardized access has been set up as of yet. It's ''essential'' to contact the appropriate personnel before using the spin coater: we want to keep our Durand access!
    
==Information==
 
==Information==
Line 14: Line 14:  
Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading is too heavy, it won't work. Use light pieces of glass/plastic!
 
Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading is too heavy, it won't work. Use light pieces of glass/plastic!
   −
Loading a spin pattern on the PWM32 means making a recipe. A recipe has 1 to 9 steps, each one with its own RPM and duration. For basic use, we only need to change 1 step for our desired speed and duration.
+
Loading a spin pattern on the PWM32 means making a recipe. A recipe has 1 to 9 steps, each one with its own RPM and duration. For basic use we only need to change 1 step for our desired speed and duration.
    
==Standard Operating Procedure==
 
==Standard Operating Procedure==
Line 21: Line 21:  
2. Turn on the yellow and black box.  
 
2. Turn on the yellow and black box.  
    +
[[File:pad.jpg|200px|thumb|right|The glorious green and red pad]]
 
3. For basic spin coating,  
 
3. For basic spin coating,  
 
   a. Press Recipe
 
   a. Press Recipe
39

edits

Navigation menu