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[[File:spinner.jpg|350px|thumb|right|Durand Spin Coater in all it's pre-1980's glory]]
[[File:spinner.jpg|350px|thumb|right|Durand Spin Coater in all it's pre-1980's glory]]
==Permissions==
==Permissions==
Anyone who wants to use the spin coater has to contact Michael Arcidiacono ({{slack-user|mixarcid}}) or one of the bio team coleads first. The spin coater is a new addition to the SSI toolset, and no standardized access has been set up as of yet. It's ''essential'' to contact the appropriate personnel before using the spin coater: we want to keep our Durand access!
Anyone who wants to use the spin coater has to contact Michael Arcidiacono ({{slack-user|mixarcid}}) or one of the bio team coleads first. The spin coater is a new addition to the SSI toolset and no standardized access has been set up as of yet. It's ''essential'' to contact the appropriate personnel before using the spin coater: we want to keep our Durand access!
==Information==
==Information==
[https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc.
[https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc.
Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading your
Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading is too heavy, it won't work. Use light pieces of glass/plastic!
Loading a spin pattern on the PWM32 means making a recipe. A recipe has 1 to 9 steps, each one with its own RPM and duration. For basic use we only need to change 1 step for our desired speed and duration.
==Standard Operating Procedure==
==Standard Operating Procedure==
2. Turn on the yellow and black box.
2. Turn on the yellow and black box.
3. For basic spin coating:
3. For basic spin coating,
a. Press Recipe
a. Press Recipe
b. Press 3: This is the recipe that we'll be using for SSI. A recipe has from 1 to 9 steps, each with a different length and RPM. For basic use, only change the first step of recipe 3.
b. Press 3: This is the recipe that we'll be using for SSI.
c. Press Step, then 1 on the keypad (to change the first step)
c. Press Step, then 1 on the keypad (to change the first step).
d. Press Step terminate and set the duration with the number pad. Then press enter.
d. Press Step terminate and set the duration with the number pad. Then press enter.
e. Press Speed and set RPMs with the number pad. Then press enter.
e. Press Speed and set RPMs with the number pad. Then press enter.
5. Turn on the power switch on the vacuum.
5. Turn on the power switch on the vacuum.
[[File:pad.jpg|200px|thumb|right|The vacuum and the green/red pad]]
6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe.
6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe.
[http://www.gaudi.ch/OpenDrop/?p=116 Open drop spin coating procedure]
[http://www.gaudi.ch/OpenDrop/?p=116 Open drop spin coating procedure]
[[Category:Biology]]