Difference between revisions of "Spin Coating"
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The spin coater is located in the Durand Building [https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc. | The spin coater is located in the Durand Building [https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc. | ||
| − | Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring | + | Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading your |
==Standard Operating Procedure== | ==Standard Operating Procedure== | ||
1. Plug in the vacuum and spinner power cords into the outlet by the fume hood. | 1. Plug in the vacuum and spinner power cords into the outlet by the fume hood. | ||
2. Turn on the yellow and black box. | 2. Turn on the yellow and black box. | ||
| − | 3. For basic spin coating, | + | 3. For basic spin coating: |
| + | a. Press Recipe | ||
| + | b. Press 3: This is the recipe that we'll be using for SSI. A recipe has from 1 to 9 steps, each with a different length and RPM. For basic use, only change the first step of recipe 3. | ||
| + | c. Press Step, then 1 on the keypad (to change the first step) | ||
| + | d. Press Step terminate and set the duration with the number pad. Then press enter. | ||
| + | e. Press Speed and set RPMs with the number pad. Then press enter. | ||
| + | 4. Center your slide in the bowl, pipet a droplet of liquid onto the center of the substrate and close the lid. If the slide is too heavy or too off-center it'll fall off and the machine will make a high pitched noise. This isn't a big problem, just recenter and try again. | ||
| + | 5. Turn on the power switch on the vacuum. | ||
| + | 6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe. | ||
| + | 7. Congratulations! You're done. Make sure you unplug/turn off everything and clean up afterwards. Again, we want to keep our access! | ||
=Useful links= | =Useful links= | ||
Revision as of 06:23, 12 November 2017
Spin coating is used to uniformly coat a flat substrate with a substance, generally oil. The substrate is placed on the spinner with a small drop of substance in the center, and then spun at high RPMs to coat. This is useful for bio team specifically, which spin coats ETFE with fluoropel to create a hydrophobic film used in electrowetting.
Spin coat for SSI
Durand has a spin coater that SSI Biology has permission to use. SOP is coming soon!
Permissions
Anyone who wants to use the spin coater has to contact Michael Arcidiacono (
@{{{display-name}}}
Information
The spin coater is located in the Durand Building (address) in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc.
Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading your
Standard Operating Procedure
1. Plug in the vacuum and spinner power cords into the outlet by the fume hood. 2. Turn on the yellow and black box. 3. For basic spin coating:
a. Press Recipe b. Press 3: This is the recipe that we'll be using for SSI. A recipe has from 1 to 9 steps, each with a different length and RPM. For basic use, only change the first step of recipe 3. c. Press Step, then 1 on the keypad (to change the first step) d. Press Step terminate and set the duration with the number pad. Then press enter. e. Press Speed and set RPMs with the number pad. Then press enter.
4. Center your slide in the bowl, pipet a droplet of liquid onto the center of the substrate and close the lid. If the slide is too heavy or too off-center it'll fall off and the machine will make a high pitched noise. This isn't a big problem, just recenter and try again. 5. Turn on the power switch on the vacuum. 6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe. 7. Congratulations! You're done. Make sure you unplug/turn off everything and clean up afterwards. Again, we want to keep our access!
Useful links
Headway Research Spin Coater User Guide