Changes

= Biology =

= Biology =

* Enzymatic DNA Synthesis Methods, '''Lead: Michael Uttmark''' {{slack-user|uttmark}}

== Enzymatic DNA Synthesis Methods ==

'''Lead: Michael Uttmark''' {{slack-user|uttmark}}

** Test commercial blocking groups for compatibility with [[Terminal Deoxynucleotidyl Transferase]]

** Test commercial blocking groups for compatibility with [[Terminal Deoxynucleotidyl Transferase]]

** Chemically synthesize nucleotides with different reversible blocking groups

** Chemically synthesize nucleotides with different reversible blocking groups

** Research purification methods for synthesized DNA  

** Research purification methods for synthesized DNA  

** Design and test your own synthesis method!

** Design and test your own synthesis method!

* Sequence Verification

 

== Sequence Verification ==

** Execute and optimize any one of our existing verification procedures--[[Polyacrylamide Gel Electrophoresis]], [[Pyrosequencing]], or [[Ligation and Sequencing]]

** Execute and optimize any one of our existing verification procedures--[[Polyacrylamide Gel Electrophoresis]], [[Pyrosequencing]], or [[Ligation and Sequencing]]

** Adapt LCMS or MALDI-TOF procedures for detecting single-base addition or determining the sequence of a sample.  

** Adapt LCMS or MALDI-TOF procedures for detecting single-base addition or determining the sequence of a sample.  

** Come up with new ways to verify single-base addition to a starting strand of DNA

** Come up with new ways to verify single-base addition to a starting strand of DNA

* Microfluidic Device Design

 

== Microfluidic Device Design ==

** Design and program an [[Electrowetting on Dielectric]] microfluidic PCB

** Design and program an [[Electrowetting on Dielectric]] microfluidic PCB

** Simulate and test how a microfluidic system would work in microgravity

** Simulate and test how a microfluidic system would work in microgravity