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==Standard Operating Procedure==
==Standard Operating Procedure==
1. Plug in the vacuum and spinner power cords into the outlet by the fume hood.
1. Plug in the vacuum and spinner power cords into the outlet by the fume hood.
2. Turn on the yellow and black box.
2. Turn on the yellow and black box.
3. For basic spin coating:
3. For basic spin coating:
a. Press Recipe
a. Press Recipe
d. Press Step terminate and set the duration with the number pad. Then press enter.
d. Press Step terminate and set the duration with the number pad. Then press enter.
e. Press Speed and set RPMs with the number pad. Then press enter.
e. Press Speed and set RPMs with the number pad. Then press enter.
4. Center your slide in the bowl, pipet a droplet of liquid onto the center of the substrate and close the lid. If the slide is too heavy or too off-center it'll fall off and the machine will make a high pitched noise. This isn't a big problem, just recenter and try again.
4. Center your slide in the bowl, pipet a droplet of liquid onto the center of the substrate and close the lid. If the slide is too heavy or too off-center it'll fall off and the machine will make a high pitched noise. This isn't a big problem, just recenter and try again.
5. Turn on the power switch on the vacuum.
5. Turn on the power switch on the vacuum.
6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe.
6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe.
7. Congratulations! You're done. Make sure you unplug/turn off everything and clean up afterwards. Again, we want to keep our access!
7. Congratulations! You're done. Make sure you unplug/turn off everything and clean up afterwards. Again, we want to keep our access!
P.S. For SSI Biology device: standard procedure for coating ETFE with fluoropel is 30s at 3000 RPM. Then cure on a hot plate: 80 degrees Celsius for 20 minutes, 150 degrees Celsius for 30 minutes.
=Useful links=
=Useful links=