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| The spin coater is located in the Durand Building [https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc. | | The spin coater is located in the Durand Building [https://www.google.com/maps/place/Durand+Building,+496+Lomita+Mall,+Stanford,+CA+94305/@37.4268696,-122.1754608,17z/data=!3m1!4b1!4m5!3m4!1s0x808fbb2aa1a2e6c7:0x150c778bd3b558cf!8m2!3d37.4268696!4d-122.1732721 (address)] in room 150. This is the lab directly to the right of the first floor entrance. The spin coater is a model PWM32-PS-CB15 Spinner System developed by Headway Research, Inc. |
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− | Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring bring gloves, micropipettes, and a small, lightweight piece of glass/plastic to spin on. | + | Make sure you're wearing safety glasses when using the spinner! Glasses are on the wall to the right when you enter the room. Bring a micropipette and a small, lightweight piece of glass/plastic to spin on. The spinner uses a vacuum to keep the slide on the rotating bit, so no special attachment is necessary. But, if your slide that you're loading your |
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| ==Standard Operating Procedure== | | ==Standard Operating Procedure== |
| 1. Plug in the vacuum and spinner power cords into the outlet by the fume hood. | | 1. Plug in the vacuum and spinner power cords into the outlet by the fume hood. |
| 2. Turn on the yellow and black box. | | 2. Turn on the yellow and black box. |
− | 3. For basic spin coating, press Recipe -> 3 -> Step -> 1 -> Step terminate | + | 3. For basic spin coating: |
| + | a. Press Recipe |
| + | b. Press 3: This is the recipe that we'll be using for SSI. A recipe has from 1 to 9 steps, each with a different length and RPM. For basic use, only change the first step of recipe 3. |
| + | c. Press Step, then 1 on the keypad (to change the first step) |
| + | d. Press Step terminate and set the duration with the number pad. Then press enter. |
| + | e. Press Speed and set RPMs with the number pad. Then press enter. |
| + | 4. Center your slide in the bowl, pipet a droplet of liquid onto the center of the substrate and close the lid. If the slide is too heavy or too off-center it'll fall off and the machine will make a high pitched noise. This isn't a big problem, just recenter and try again. |
| + | 5. Turn on the power switch on the vacuum. |
| + | 6. Find the green/red floor button and press the green button to start the spin. Pressing the red button is an emergency brake. There are two sets of green/red buttons: use the newer one. While spinning you can use the two buttons next to the speed arrows to change the RPMs; however, it's suggested that all your spins are preprogrammed in the recipe. |
| + | 7. Congratulations! You're done. Make sure you unplug/turn off everything and clean up afterwards. Again, we want to keep our access! |
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| =Useful links= | | =Useful links= |